Oxidative imbalance in Sickle Cell Disease

About author
Nnamah, N.K,Igboh, N. M; Onwubiko, D.,Chigbu, L.N., Agomuo,E.N.,Onyesom,C.A,Maduagwun,C.A, Iheanacho,K,M..E Emuchey,C.I.
Department of chemical pathology, College of Medicine and Health Sciences, Nnamdi Azikiwe University Teaching Hospital,  Nnewi. Nigeria.
College of Medicine and Health Sciences, Abia State University Uturu. Nigeria.
Department of Biochemistry, Faculty of Sciences. Imo State University, Owerri, Nigeria.
Department of Medical Biochemistry Delta State University, Abraka, Nigeria.
Department of Pharmacology, College of Medicine and Health Sciences, Abia State University Uturu. Nigeria
Department of Biochemistry, Faculty of Sciences. Federal University of Technology Owerri, Nigeria.

Abstract
Oxidative defense imbalance in Sickle Cell Disease was evaluated using enzymatic (Lactate Dehydrogenase) and nonenzymatic (Uric acid and Ascorbic Acid (vit C)) indicators. The study was carried out using eight patients with sickle cell disease in steady state, seven patients with Sickle Cell Disease in crisis. Twenty patients with AS haemoglobin and another twenty of normal haemoglobin (AA) served as Control. All groups have age range between 10-30 years comprising of both sex. All analysis was based on calorimetric assay. Both serum uric acid and LDH concentrations were significantly higher in sickle cell patients in crisis compared to patients with sickle cell disease in steady state and the healthy patients. ( Lactate Dehydrogenase (U/L) AA 288.05 ± 8.53, Sicklers 383.88 ±12.59 and Sicklers In crisis 511.44 ± 14.56., Uric acid (uMol/L). AA   321.80 +2.75 Sicklers  339.48. ±4.60   Sicklers in crisis 470.66. ±9.23 (P<0.05).However, the level of Ascorbic Acid (vit C) was remarkably reduced in Sicklers cell patients in crisis (P<0.05).  This study has stressed further the importance of antioxidant in the management of sickle cell disease.
Key words: Lactate Dehydrogenase, Uric acid, Ascorbic Acid, Sickle Cell Disease